FIGURE 6.

Effect of AO extracts on SIRT1/Nrf2 signaling pathway. AO methanol extract treatment increased the SIRT1 (A), Nrf2 expression (B) and antioxidant-related target genes (C) in Neuro-2a cells when compared to the untreated control. Samples were treated with AO extracts for 48 h. Whole-cell lysates were subjected to western blot analysis of the SIRT1 and Nrf2 levels after AO extract treatment. β-actin was used as an endogenous loading control for western blot assay and internal control for RT-PCR assay. All data were normalized to endogenous control levels and the results are expressed as the means ± SEM of independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, compared to the untreated control.