FIGURE 8.

Effect of AO extracts on Ten-4-mediated neurite outgrowth. AO methanol extract treatment increased expression level of Ten-4 mRNA (A) and protein (B). AO methanol extract failed to induced neurite length (C) and neurite-bearing cells (D) in siTen-4-Neuro-2a cells. Results were confirmed by GAP43 mRNA expression (E). Cell morphology of Neuro-2a cells was observed under a microscope at 10× magnification (F). Samples were treated with AO extracts for 48 h. Whole-cell lysates were subjected to western blot analysis at the Ten-4 level after AO extract treatment. β-actin was used as endogenous loading control for western blot assay and internal control for RT-PCR assay. All data were normalized to 10% FBS control levels in siCont-Neuro-2a cells and the results are expressed as the means ± SEM of independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared to the 1% FBS control.