Fig. 6.

Mechanical allodynia, eye closing ratio, and anxiety of DED animals treated with 10 μM capsazepine or vehicle. a Corneal mechanical sensitivity measured with von Frey filaments. b Images and quantification of the spontaneous eye closing ratio calculated by measuring the width/height ratio. c Time spent in the open arms (s) in the elevated plus maze test. d Time spent in the white zone (%) in the black and white box test by DED animals treated with 10 μM capsazepine or vehicle. Total number of mice = 20. Number of mice included in the analysis = 20. DED animals treated with vehicle and 10 μM capsazepine: n = 10. All tests were conducted on d21. *P < 0.05, **P < 0.01 relative to DED treated with vehicle. Results are expressed as the mean ± SEM. For statistical analysis, the Kolmogorov–Smirnov test was performed followed by a parametric t-test (von Frey and spontaneous eye closure ratio) or the nonparametric Kruskal-Wallis test (elevated plus maze test and black and white test) using GraphPad Prism version 7.00 (GraphPad Software, La Jolla, CA, USA)