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In vitro 3D spheroids. (A) Viability of BxPC-3 cells in 3D spheroid cultures under normoxic and hypoxic (2% O2) conditions. (B) Image of a single spheroid pre-treatment. (C) Spheroid after 72-hour treatment with iRGD-polymersomes, using live (green) and dead (red) staining. * p-value <0.05, n = 8.
