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. 2021 May 12;1(2):100011. doi: 10.1016/j.crmeth.2021.100011
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-Spike-RBD antibody Novus Biologicals NBP2-90980
Anti-nucleocapsid antibody ThermoFisher Scientific Cat# PA5-81794; RRID: AB_2788968
Goat anti-human IgG HRP antibody Millipore Sigma Cat# AP113P; RRID: AB_92443
Goat anti-rabbit IgG HRP Bio-Rad Cat# 170-6515; RRID: AB_11125142

Chemicals, reagents and recombinant protein

Blue dyed polystyrene latex beads, 0.8 μm Sigma Aldrich L1398
biotin using EZ-Link Sulfo-NHS-LC-LC-Biotin ThermoFisher Scientific A35358
ChonBlock ELISA blocking and antibody dilution buffer Chondrex Inc 9068
TMB substrate (3,3’,5,5’-Tetramethylbenzidine) ThermoFisher Scientific N301
Spike Receptor Binding Domain (RBD) (14μM) ThermoFisher Scientific RP-87678
Nucleocapsid (full length) (5μM) RayBiotech 230-01104
ACE2 protein This Study

Software

Qupath https://qupath.github.io/ v0.1.2