Antibodies |
|
Anti-Spike-RBD antibody |
Novus Biologicals |
NBP2-90980 |
Anti-nucleocapsid antibody |
ThermoFisher Scientific |
Cat# PA5-81794; RRID: AB_2788968
|
Goat anti-human IgG HRP antibody |
Millipore Sigma |
Cat# AP113P; RRID: AB_92443
|
Goat anti-rabbit IgG HRP |
Bio-Rad |
Cat# 170-6515; RRID: AB_11125142
|
|
Chemicals, reagents and recombinant protein
|
|
Blue dyed polystyrene latex beads, 0.8 μm |
Sigma Aldrich |
L1398 |
biotin using EZ-Link Sulfo-NHS-LC-LC-Biotin |
ThermoFisher Scientific |
A35358 |
ChonBlock ELISA blocking and antibody dilution buffer |
Chondrex Inc |
9068 |
TMB substrate (3,3’,5,5’-Tetramethylbenzidine) |
ThermoFisher Scientific |
N301 |
Spike Receptor Binding Domain (RBD) (14μM) |
ThermoFisher Scientific |
RP-87678 |
Nucleocapsid (full length) (5μM) |
RayBiotech |
230-01104 |
ACE2 protein |
This Study |
|
|
Software |
|
Qupath |
https://qupath.github.io/ |
v0.1.2 |