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. 2021 May 11;22:339. doi: 10.1186/s12864-021-07649-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Median expression profiles of DETs assigned to Iso-MaSigPro clusters. Iso-MaSigPro grouped the differentially expressed transcripts (DETs) into six clusters. DETs of cluster 1, 4 and 6 were especially highly expressed in old queens, while those of cluster 3 and 5 characterized middle-aged queens and those of cluster 2 young queens. The expression values correspond to normalized counts (see Methods). The youngest queen (age: 2 years) was taken as time step zero and each of the subsequent older queens (based on chronological age) were considered to be one time step older. One age class (time step 8; age: 10 years) consisted of two samples