Table 4.
Significantly enriched biological functions of the DEGs
| Group contrast | Ontology source | Biological function | Terms per group (%) |
|---|---|---|---|
| A) LOW vs HIGH | GO_Biological Process | transmembrane transport | 14.23 |
| GO_Molecular Function | ATP binding | 7.11 | |
| GO_Biological Process | male gamete generation | 6.3 | |
| GO_Cellular Component | presynapse | 5.89 | |
| GO_Biological Process | ion channel activity | 5.08 | |
| GO_Biological Process | regulation of macromolecule metabolic process | 6.67 | |
| GO_Biological Process | ion transport | 3.05 | |
| GO_Cellular Component | sodium channel complex | 2.89 | |
| others | 48.78 | ||
| B) LOW vs NORM | GO_Biological Process | flagellated sperm motility | 38.46 |
| GO_Biological Process | inorganic anion transmembrane transport | 26.92 | |
| GO_Cellular Component | postsynaptic density | 7.69 | |
| others | 26.93 | ||
| C) Interaction effect of prenatal nutrition and sex (PreNxsex) | GO_Cellular Component | dynein complex | 21.43 |
| GO_Biological Process | inorganic molecular entity transmembrane transporter activity | 20.00 | |
| GO_Biological Process | cell development | 8.57 | |
| GO_Biological Process | ion transmembrane transporter activity | 7.14 | |
| GO_Molecular Function | motor activity | 7.14 | |
| others | 35.72 | ||
| D) LOW-HCHF vs NORM-CONV | GO_Biological Process | leukocyte activation | 35.32 |
| GO_Biological Process | regulation of interleukin-6 production | 10.04 | |
| GO_Molecular Function | cell adhesion molecule binding | 8.92 | |
| GO_Biological Process | positive regulation of myeloid cell differentiation | 7.81 | |
| KEGG | Cholesterol metabolism | 3.35 | |
| others | 34.56 | ||
| E) HIGH-HCHF vs NORM-CONV | GO_Biological Process | leukocyte activation | 18.43 |
| GO_Biological Process | regulation of immune system process | 6.95 | |
| GO_Biological Process | positive regulation of interleukin-1 production | 4.02 | |
| GO_Biological Process | regulation of leukocyte mediated immunity | 3.15 | |
| GO_Biological Process | positive regulation of leukocyte mediated immunity | 3.07 | |
| others | 64.38 | ||
| F) HCHF vs CONV | |||
| Upregulated | GO_Biological Process | transmembrane transporter activity | 26.27 |
| GO_Biological Process | regulation of postsynaptic membrane potential | 9.91 | |
| GO_Biological Process | regulation of natural killer cell mediated immunity | 6.68 | |
| others | 57.14 | ||
| Downregulated | GO_Biological Process | regulation of immune system process | 15.35 |
| GO_Biological Process | inflammatory response | 11.68 | |
| GO_Cellular Component | proton-transporting V-type ATPase, V1 domain | 3.00 | |
| GO_Biological Process | actin cytoskeleton organization | 3.84 | |
| GO_Biological Process | cell differentiation involved in kidney development | 0.25 | |
| others | 65.88 |