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Buffy coat wash solution (temperature ∼20°C)
| Reagent | Final concentration | Stock concentration | Amount (mL) |
|---|---|---|---|
| Sterile DPBS | n/a | n/a | 450 |
| FBS | 10% v/v | n/a | 50 |
Excess solutions that contain FBS should be stored at 4°C. All other reagents in this protocol are listed in the key resources table.
