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. 2021 May 7;14:3051–3062. doi: 10.2147/OTT.S294136

Figure 2.

Figure 2

Effects of IFNγ and kinase inhibitors on PD-L1 expression in the MSI and MSS cell lines. (A–C) PD-L1 expression was measured by flow cytometry in HCT15 (the upper line) and SW620 (the lower line) cell lines 12 h after treatment with 100 IU/mL IFNγ, with 1 μmol/L wortmannin (AKT inhibitor), 50 μmol/L PD98059 (ERK1/2 inhibitor), or 50 μmol/L fludara (STAT1 inhibitor). DMSO was used as a vehicle and negative control (*P < 0.05 vs cells treated with IFNγ and DMSO). (D) Expression levels of STAT1, p-STAT1, STAT3 and p-STAT3 in MSI cell lines (HCT116 and HCT15) as well as MSS cell lines (SW480 and SW620) with 100 IU/mL IFNγ for 15 min and 12 h, respectively.