Table 2.
Summary of statistical tests
| Post hoc comparisons | ||||||||
|---|---|---|---|---|---|---|---|---|
| Figure | Test | Results | p value | 95.00% CI of diff. | Mean 1 | Mean 2 | Sig. | |
| 1B | Two-way ANOVA | Interaction: F(50,816) = 0.1519 | >0.9999 | |||||
| Frequency: F(50,816) = 31.82 | <0.0001 | |||||||
| Genotype: F(1,816) = 2.519 | 0.1129 | |||||||
| 1D | Two-way ANOVA | Interaction: F(50,765) = 1.637 | 0.0043 | 1 Hz | −85.94 to −8.531 | 134.17 | 181.41 | ** |
| Frequency: F(50,765) = 47.27 | <0.0001 | 2 Hz | −99.88 to −22.47 | 146.01 | 207.19 | **** | ||
| Genotype: F(1,765) = 18.97 | <0.0001 | 3 Hz | −95.39 to −17.98 | 113.21 | 169.89 | **** | ||
| 4 Hz | −85.79 to −8.374 | 87.19 | 134.27 | ** | ||||
| 1F | Two-way ANOVA | Interaction: F(50,561) = 2.252 | <0.0001 | 1 Hz | −227.3 to −66.59 | 214.79 | 361.74 | **** |
| Frequency: F(50,561) = 30.41 | <0.0001 | 2 Hz | −245.6 to −84.88 | 220.65 | 385.89 | **** | ||
| Genotype: F(1,561) = 29.53 | <0.0001 | 3 Hz | −199.9 to −39.24 | 157.28 | 276.87 | **** | ||
| 4 Hz | −162.9 to −2.170 | 109.55 | 192.08 | * | ||||
| 1H | Two-way ANOVA | Interaction: F(50,408) = 1.149 | 0.2358 | 1 Hz | −129.1 to −21.47 | 191.40 | 266.67 | *** |
| Frequency: F(50,408) = 41.52 | <0.0001 | 2 Hz | −128.2 to −20.64 | 184.40 | 258.83 | *** | ||
| Genotype: F(1,408) = 13.53 | 0.0003 | |||||||
| 1-1B | Unpaired t test with Welch’s correction | t = 10.40, df = 4.063; 95% CI: −1.208 to −0.7016 | 0.0004 | |||||
| 1-2B | Two-way ANOVA | Interaction: F(50,867) = 0.9298 | 0.6134 | 1 Hz | −114.5 to −9.244 | 142.99 | 204.87 | ** |
| Frequency: F(50,867) = 29.69 | <0.0001 | 2 Hz | −124.7 to −19.43 | 160.32 | 232.38 | *** | ||
| Genotype: F(1,867) = 5.405 | 0.0203 | |||||||
| 1-2D | Two-way ANOVA | Interaction: F(50,408) = 0.7116 | 0.9303 | 1 Hz | −134.8 to −34.49 | 114.52 | 199.17 | **** |
| Frequency: F(50,408) = 18.65 | <0.0001 | |||||||
| Genotype: F(1,408) = 0.7579 | 0.3845 | |||||||
| 2B | Two-way ANOVA | Interaction: F(50,765) = 0.2408 | >0.9999 | |||||
| Frequency: F(50,765) = 20.50 | <0.0001 | |||||||
| Genotype: F(1,765) = 0.4037 | 0.5254 | |||||||
| 2D | Two-way ANOVA | Interaction: F(50,765) = 1.665 | 0.0033 | 1 Hz | −1193 to −269.8 | 1747.29 | 2478.75 | **** |
| Frequency: F(50,765) = 38.40 | <0.0001 | 2 Hz | −1232 to −308.9 | 1407.57 | 2178.16 | **** | ||
| Genotype: F(1,765) = 15.16 | 0.0001 | 3 Hz | −1050 to −126.7 | 876.49 | 1464.83 | ** | ||
| 2F | Two-way ANOVA | Interaction: F(50,561) = 2.855 | <0.0001 | 1 Hz | −2614 to −986.7 | 2801.60 | 4602.08 | **** |
| Frequency: F(50,561) = 52.60 | <0.0001 | 2 Hz | −2674 to −1047 | 3021.36 | 4881.72 | **** | ||
| Genotype: F(1,561) = 21.59 | <0.0001 | 3 Hz | −2135 to −507.5 | 2254.47 | 3575.70 | **** | ||
| 4 Hz | −1687 to −59.74 | 1626.22 | 2499.73 | * | ||||
| 2H | Two-way ANOVA | Interaction: F(50,255) = 0.8056 | 0.8194 | 1 Hz | −2861 to −279.7 | 2253.00 | 3823.60 | ** |
| Frequency: F(50,255) = 13.37 | <0.0001 | 2 Hz | −2768 to −186.7 | 2214.00 | 3691.60 | ** | ||
| Genotype: F(1,255) = 10.13 | 0.0016 | |||||||
| 2-1B | Two-way ANOVA | Interaction: F(50,867) = 2.797 | <0.0001 | −1659 to −404.5 | 2472.56 | 3504.52 | **** | |
| Frequency: F(50,867) = 49.22 | <0.0001 | −2014 to −759.1 | 2199.18 | 3585.74 | **** | |||
| Genotype: F(1,867) = 26.10 | <0.0001 | 3 Hz | −1828 to −572.9 | 1373.97 | 2574.32 | **** | ||
| 4 Hz | −1512 to −257.5 | 965.80 | 1850.69 | *** | ||||
| 5 Hz | −1265 to −9.782 | 825.34 | 1462.54 | * | ||||
| 2-1D | Two-way ANOVA | Interaction: F(50,306) = 1.048 | 0.3937 | 1 Hz | −733.2 to −164.3 | 1002.67 | 1451.40 | **** |
| Frequency: F(50,306) = 46.53 | <0.0001 | 2 Hz | −605.8 to −36.91 | 1032.67 | 1354.00 | * | ||
| Genotype: F(1,306) = 9.863 | 0.0019 | |||||||
| 3B, dark cycle | Unpaired t test with Welch’s correction | t = 2.612, df = 10.98; 95% CI: 0.1150–1.347 | 0.0242 | |||||
| 3B, light cycle | t = 2.480, df = 11.70; 95% CI: 0.08133–1.287 | 0.0294 | ||||||
| 3D, dark cycle | Unpaired t test with Welch’s correction | t = 2.296, df = 8.633; 95% CI: 0.01022–2.409 | 0.0485 | |||||
| 3D, light cycle | Unpaired t test with Welch’s correction | t = 2.116, df = 8.641; 95% CI: −0.1092–2.980 | 0.0647 | |||||
| 4A, latency clonus | Unpaired t test | t = 0.08745, df = 21; 95% CI: −138.9–127.7 | 0.9311 | |||||
| 4A, latency seizure | Unpaired t test with Welch’s correction | t = 0.4379, df = 9.041; 95% CI: −481.5–325.2 | 0.6717 | |||||
| 4A, recovery | Unpaired t test | t = 0.6691, df = 21; 95% CI: −1127–2197 | 0.5107 | |||||
| 4B, latency clonus | Unpaired t test | t = 0.4538, df = 28. 95% CI: −47.05–29.98 | 0.6534 | |||||
| 4B, latency seizure | Unpaired t test | t = 0.5004, df = 25; 95% CI: −13.37–21.95 | 0.6211 | |||||
| 4B, recovery | Unpaired t test | t = 1.693, df = 30; 95% CI: −18.96–202.6 | 0.1009 | |||||
| 4C, latency clonus | Unpaired t test | t = 1.605, df = 25; 95% CI: −18.48–2.295 | 0.1211 | |||||
| 4C, latency seizure | Unpaired t test | t = 0.7429, df = 23; 95% CI: −13.22–6.234 | 0.4651 | |||||
| 4C, recovery | Unpaired t test | t = 2.523, df = 19; 95% CI: 237.9–2552 | 0.0207 | |||||
| 4-1A, immobility | Unpaired t test | t = 0.1999, df = 14; 95% CI: −39.49–32.76 | 0.8444 | |||||
| 4-1A, recovery | Unpaired t test | t = 1.531, df = 14; 95% CI: −47.37–283.8 | 0.148 | |||||
| 4-1B, immobility | Unpaired t test | t = 2.074, df = 22; 95% CI: −69.50 to −0.002622 | 0.05 | |||||
| 4-1B, recovery | Unpaired t test | t = 3.054, df = 22; 95% CI: 113.7–595.3 | 0.0058 | |||||
| 4-1C, immobility | Unpaired t test | t = 0.5781, df = 26; 95% CI: −22.82–40.68 | 0.5682 | |||||
| 4-1C, recovery | Unpaired t test | t = 0.2767, df = 26; 95% CI: −57.80–44.09 | 0.7842 | |||||
| 5C, 30 min: cortical | Unpaired t test | t = 0.1210, df = 5; 95% CI: t = 0.1210, df = 5 | 0.9084 | |||||
| 5C, 30 min: hippocampal | Unpaired t test | t = 0.07701, df = 5; 95% CI: −22.03–20.75 | 0.9416 | |||||
| 5C, 3 h: cortical | Unpaired t test | t = 3.039, df = 5; 95% CI: 4.279–51.22 | 0.0288 | |||||
| 5C, 3 h hippocampal | Unpaired t test | t = 3.098, df = 5; 4.972–53.41 | 0.0269 | |||||
| 6C | Unpaired t test | t = 2.806, df = 13; 95% CI: −5.558 to −0.7225 | 0.0149 | |||||
*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.