Table 1.

Summary of mixed linear models

Source	DF	F	p	Variance component	Estimate	SE	95% lower	95% upper	p
LS
Housing	1, 22.8	0.28	0.60	Cage number	−4.18	4.95	−13.89	5.53	0.40
Drug	2, 22.8	15.98	<0.0001***	IHC run	7.83	9.45	−10.70	26.36	0.41
Housing × drug	2, 22.8	4.84	0.02*	Residual	33.51	8.33	21.73	58.40
BNST
Housing	1, 14.5	7.23	0.02*	Cage number	3.15	6.84	−10.25	16.55	0.65
Drug	2, 14.5	52.63	<0.0001***	IHC run	2.80	4.31	−5.64	11.24	0.52
Housing × drug	2, 14.5	0.49	0.62	Residual	20.98	6.70	12.23	44.13
mPOA
Housing	1, 20.5	6.67	0.02*	Cage number	57.24	24.33	9.56	104.93	0.02*
Drug	2, 20.5	25.34	<0.0001***	IHC run	34.86	40.78	−45.08	114.80	0.39
Housing × drug	2, 20.5	1.96	0.17	Residual	28.66	10.54	15.59	69.15
PVN
Housing	1, 19.1	10.12	0.005**	Cage number	21.13	12.54	−3.44	45.70	0.09
Drug	2, 19.1	40.99	<0.0001***	IHC run	13.95	17.01	−19.39	47.29	0.41
Housing × drug	2, 19.1	8.25	0.003**	Residual	25.61	8.42	14.71	55.33
AH
Housing	1, 21.2	0.18	0.67	Cage number	0.11	4.80	−9.29	9.51	0.98
Drug	2, 21.2	6.60	0.001**	IHC run	4.92	6.33	−7.48	17.32	0.44
Housing × drug	2, 21.2	1.91	0.17	Residual	24.55	6.49	15.53	44.58
VMH
Housing	1, 21.5	2.52	0.13	Cage number	7.18	2.50	2.28	12.08	0.004**
Drug	2, 21.5	14.47	<0.0001***	IHC run	0.49	1.01	−1.48	2.46	0.63
Housing × drug	2, 21.5	0.06	0.94	Residual	1.48	0.56	0.79	3.68
PAG
Housing	1, 22.5	6.11	0.02*	Cage number	32.71	11.90	9.39	56.04	0.01**
Drug	2, 22.5	0.91	0.42	IHC run	5.48	8.02	−10.24	21.21	0.49
Housing × drug	2, 22.5	1.45	0.26	Residual	10.71	3.87	5.88	25.36

Statistically significant main effects and interactions indicated with asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.