Skip to main content
. 2020 Sep 24;31(4):450–462. doi: 10.1038/s41422-020-00411-7

Fig. 2. Lrp6 deficiency induces cell cycle activation in mouse hearts at the juvenile and adult stages in vivo.

Fig. 2

a Generation of the mouse line with Lrp6-CKO. Top, schematic diagram. Bottom, qRT-PCR examination of Lrp6 mRNA levels in the cardiomyocytes (CMs) of the Lrp6-CKO mice. n = 4 mice per group. b Immunostaining showing co-staining of pH3-S10, cTnT and DAPI in the Cre+/− and Lrp6-CKO hearts at P7d and P14d, respectively. The graph shows the number of pH3-S10+cTnT+ nuclei. n = 3–5 mice per group. Scale bar = 100 µm. c, d Immunostaining showing EdU+cTnT+DAPI+ and pH3-S10+cTnT+DAPI+ cardiomyocytes in the Cre+/− and Lrp6-CKO hearts at 10 weeks. The graph shows the percentage of EdU+cTnT+DAPI+ cells and the number of pH3-S10+cTnT+ DAPI+ cells. The results of quantitative analysis represent 3 to 6 independent samples per group (three sections per heart). Scale bar = 100 µm. e Quantifications of proliferating CMs stained positive for EdU in dissociated CMs from adult Cre+/− and Lrp6-CKO mice injected with EdU substrate. Approximately 5000–7000 cardiomyocytes were counted from 3 mice per group. f, g The nucleation and ploidy assessment of dissociated CMs from adult Cre+/− and Lrp6-CKO mice. 2n, diploid, 4n, tetraploid, 4n+, polyploid. MNCMs, mononucleated CMs. n = 4 mice per group. h Quantifications of the percentage of MNDCMs within the EdU+ CMs population from adult Cre+/− and Lrp6-CKO mice. MNDCMs mononucleated diploid CMs. EdU-positive cardiomyocytes were counted from 3 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. n.s. not significant. Data are presented as means ± SD.