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. 2021 Apr 28;9:650067. doi: 10.3389/fcell.2021.650067

FIGURE 2.

FIGURE 2

β-arrestin1 regulates the EMT of CRC cells. (A,B) The expression of β-arrestin1 was detected in six CRC cell lines by reverse transcription quantitative PCR. β-arrestin1 had a higher expression level in LoVo and HCT-116 cells, ∗P < 0.05 vs. the other four established human CRC cell lines HCT-8, Caco-2, SW620 and RKO. (C) The mRNA expression of β-arrestin1 in shRNA/β-arrestin1 and over/β-arrestin1 groups was detected by reverse transcription PCR. ∗P < 0.05; ∗∗P < 0.01 vs. HCT-116-vector or LoVo-vector cells. (D–E) Western blotting detected the protein expression of β-arrestin1 in shRNA/β-arrestin1 and over/β-arrestin1 groups in HCT-116 or LoVo cells. ∗∗∗P < 0.001 vs. HCT-116-vector or LoVo-vector cells. (F–I) Expression levels of epithelial or mesenchymal markers in HCT-116 or LoVo cells were analyzed by western blotting. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001, vs. HCT-116-vector or LoVo-vector cells. EMT, epithelial-to-mesenchymal transition; CRC, colorectal cancer; shRNA, short hairpin RNA.