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. 2021 Apr 28;9:650067. doi: 10.3389/fcell.2021.650067

FIGURE 4.

FIGURE 4

β-arrestin1 promotes the migration of CRC cells. (A) HCT-116 or LoVo cells transfected with shRNA/β-arrestin1 or over/β-arrestin1 lentivirus were used in the Transwell experiment. Typical images of cells migrating in a transwell migration chamber were captured and quantitatively measured. *P < 0.05; **P < 0.01 vs. HCT-116-vector or LoVo-vector cells. (B) shRNA/β-arrestin1 or over/β-arrestin1 cells were cultured to confluence at 4.5 × 105 cells on 6-well plates. Representative images and a bar graph of the wound-healing assay are presented. The black line was used to mark the ranges of the scratches. *P < 0.05 vs. HCT-116-vector or LoVo-vector cells. (C,D) MMP2 and MMP9 were examined via western blotting. *P < 0.05; **P < 0.01; ***P < 0.001 vs. HCT-116-vector or LoVo-vector cells. CRC, colorectal cancer; shRNA, short hairpin RNA.