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. 2021 Apr 28;9:650067. doi: 10.3389/fcell.2021.650067

FIGURE 7.

FIGURE 7

β-arrestin1 regulates CRC EMT and metastasis in vivo. (A) Fluorescence intensity was evaluated in eight mice of each group at days 28, 33, 38 and 43 after tail vein injection. *P < 0.05 vs. HCT-116-vector group. (B–E) The lung metastasis tissues were excised after day 43, and the number of metastatic lesions was counted. *P < 0.05 vs. HCT-116-vector group. The survival curve of animals injected with HCT-116/luc or shRNA/β-arrestin1-HCT-116/luc or over/β-arrestin1-HCT-116/luc cells. *P < 0.05; **P < 0.01 vs. HCT-116-vector group. Representative hematoxylin and eosin staining of tumor cytostructure and cytomorphology analysis. (F) Immunohistochemistry detected the expression levels of β-arrestin1, E-cadherin, Vimentin and Snail in three groups. (G,H) The protein expression levels of E-cadherin, N-cadherin, Snail and Vimentin were examined via western blotting. *P < 0.05; **P < 0.01, ***P < 0.001 vs. HCT-116-vector group.