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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Adv Ther (Weinh). 2021 Jan 20;4(4):2000244. doi: 10.1002/adtp.202000244

Figure 2. OBOC peptide library screen for dg-Bcan-binding peptides yields 7 candidates.

Figure 2.

a) Schematic of the brevican protein, Bcan (top) and its deglycosylated isoform, dg-Bcan (bottom). The dg-Bcan isoform lacks the majority of N- and O-linked carbohydrates (including chondroitin sulfate chains), thus exposing specific epitopes that are masked in the glycosylated isoform. Red box denotes dg-Bcan epitope targeted for screening. b) Schematic of magnetic-activated OBOC library screen with a microfluidic sorter. c,d) Western blot analysis and immunofluorescence images showing astrocytes, U87 (empty vector) and Bcan-overexpressing U87 cells. e) Schematic of the secondary cell-based screen.