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. 2021 Apr 15;12:655320. doi: 10.3389/fphar.2021.655320

FIGURE 6.

FIGURE 6

Effect of pregnancy-related hormones (PRH) on protein concentrations of UGT1A4 and other key UGT1A isoforms in SCHH. Following 72 h of PRH exposure, UGT1A4 protein concentrations were quantified by quantitative targeted absolute proteomics in SCHH membrane-associated protein fractions isolated from three donors (HC3-26, HU1880, and HU8284). (A) UGT1A4 absolute protein concentrations in the DMSO and PRH cocktail (CKTL) groups were compared separately in donor HC3-26 (mean: n = 2 per group) and donors HU1880 and HU8284 (mean ± SEM: n = 3–4 per group; *p < 0.05 vs. DMSO, ^p < 0.05 1 vs. 10 µM). Comparisons of PRH CKTL effects on UGT1A3, UGT1A6, and UGT1A9 concentrations within each hepatocyte donor are provided in Supplementary Figure S3. (B) UGT1A4, (C) UGT1A3, (D) UGT1A6, and (E) UGT1A9 protein concentrations were expressed relative to vehicle control (DMSO) within each hepatocyte donor, and then combined for comparison across groups. Open bars represent 1 µM CKTL (mean ± SEM: n = 3 donors per group) and 1 µM for the individual hormones (mean: n = 2 donors per group). Solid bars represent 10 µM CKTL and 10 µM for the individual hormones (mean ± SEM: n = 3 donors per group). *p < 0.05 vs. DMSO, ^p < 0.05 1 vs. 10 µM. Comparison of individual PRH effects on UGT1A4 levels within each hepatocyte donor is provided in Supplementary Figure S2C.