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. 2020 Aug 11;42(5):767–779. doi: 10.1038/s41401-020-0488-1

Fig. 1. Effects of carnosine on the viability, colony formation, and apoptosis of U87 and U251 cells cultured under normoxic and hypoxic conditions.

Fig. 1

a U87 and U251 cells were exposed to carnosine at different conditions (25, 50, and 75 mM) for 48 h, or 50 mM carnosine for different time periods (24, 48, and 72 h). Cell viability was determined by MTT assay. b Colony formation of cells treated with 50 mM carnosine for 14 days under normoxic and hypoxic conditions was assessed. Clone-formation inhibition rate = (a−b)/a × 100%, where a = number of clones under normoxia or hypoxia without carnosine treatment and b = number of clones under normoxia or hypoxia with carnosine treatment. c Under normoxic and hypoxic conditions, cells were treated with 50 mM carnosine for 48 h, after which flow cytometry was used to detect the apoptosis level. Data are expressed as the mean ± SD. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. the control group or normoxia group.