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. 2020 Aug 11;42(5):767–779. doi: 10.1038/s41401-020-0488-1

Fig. 5. Carnosine combined with Gln deprivation inhibited the growth of U87 and U251 cells and induced apoptosis.

Fig. 5

U87 cells (a) and U251 cells (b) were treated with Gln deprivation, 50 mM carnosine, or both for different time periods (24, 48, and 72 h) under normoxic and hypoxic conditions. Cell viability was measured by MTT assay, and the proliferation inhibition rate was calculated as (absorbance of the control group−absorbance of the treatment group)/absorbance of the control group × 100%. c Under normoxia, cells were deprived of Gln or treated with 50 mM carnosine for 48 h, after which the GS level was detected by Western blot assay. d Under normoxia, cells were deprived of Gln or treated with 50 mM carnosine for 48 h, after which flow cytometry was used to detect the apoptosis level. Mean ± SD. n = 3–5. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. 0 mM Gln treatment, 4 mM Gln + Car treatment, 4 mM Gln treatment, or 0 mM Gln treatment.