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. 2020 Aug 11;42(5):767–779. doi: 10.1038/s41401-020-0488-1

Fig. 6. The inhibitory effect of carnosine on the proliferation and metastasis of U87 and U251 cells was partly achieved by inhibiting Gln metabolism.

Fig. 6

A lentivirus system and puromycin selection were used to establish GS cell lines with low stable GS expression (shGS) and control cell lines (NC). Western blot experiments (a) were used to identify GS protein expression in the cell lines; real-time PCR (b) was used to identify GS transcription levels. Under conditions of exogenous Gln deprivation, shGS cells were treated with 50 mM carnosine, and cell viability was tested by MTT assays (c). Transwell assays (d) were used to detect cell migration and invasion capacity. Scale bar = 100 μm. The results are expressed as the mean ± SD. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. NC or shGS group.