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. 2021 May 12;11:10143. doi: 10.1038/s41598-021-89575-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of purified complex I from P. denitrificans. (a) K_M curve for DQ and Q₁ substrates for a typical sample of soluble complex I. The K_M values for DQ and Q₁ are 169 ± 15 and 124 ± 8 µM, respectively (± S.E. of the fit). (b) Piericidin A and rotenone IC₅₀ titration curves for soluble complex I, using DQ as the substrate. The IC₅₀ values of piericidin A and rotenone are 72 ± 7 nM and 2226 ± 185 nM, respectively (± S.E. of the fit).