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. 2021 May 12;12:2745. doi: 10.1038/s41467-021-22961-z

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

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Fig. 6 — a One-way ANOVA comparison performed on Log2 transformed RPKM values of Bmal1^fl/fl and Bmal1^ΔHep liver on day 5 of TRF at ZT0 or ZT12 as indicated, comparing resistant groups (Bmal1^fl/fl NF0 and Bmal1^fl/fl DF12) vs. susceptible groups (Bmal1^fl/fl NF12, Bmal1^fl/fl DF0 and all Bmal1^ΔHep groups). Relative expression for genes that were expressed in at least one animal, a FDR < 0.2, and two-fold changed between resistant vs. susceptible groups (232) are shown in the Heatmap indicating relative expression (RPKM-mean RPKM/standard deviation) for each gene. b Core analysis using IPA of genes that were expressed in at least one animal, a FDR < 0.2, and two-fold changed (232) in a one-way ANOVA comparison performed on Log2 transformed RPKM values of Bmal1^fl/fl and Bmal1^ΔHep liver on day 5 of TRF at ZT0 or ZT12 as indicated, comparing resistant groups (Bmal1^fl/fl NF0 and Bmal1^fl/fl DF12) vs. susceptible groups (Bmal1^fl/fl NF12, Bmal1^fl/fl DF0 and all Bmal1^ΔHep groups). Top 10 canonical pathways are shown. N = 4. c Clinical score of day-time and night-time fed Fxr^ΔHep (solid line) or Fxr-floxed control female mice (dotted line) following 20 mg/kg LPS i.p. stimulation at ZT0 on day 5 of TRF. Statistical analysis using two-way ANOVA and Sidak’s multiple comparison. Fxr^ΔHep vs. Fxr^fl/fl DF: Interaction F_(17,234) = 0.1507, p > 0.9999, time F_(17,234) = 4.362, p < 0.0001, group F_(1,234) = 0.08568, p = 0.77. Fxr^ΔHep vs. Fxr^fl/fl NF_: Interaction F_(17,198) = 0^.1719, p > 0.9999, time F_(17,198) = 1.591, p = 0.693, group F_(1,198) = 12.69, p = 0.0005. Fxr^ΔHep DF vs. NF: Interaction F_(17,180) = 0.1478, p > 0.9999, time F_(17,180) = 2.754, p = 0.0004, group F_(1,180) = 0.4948, p = 0.4827. Fxr^fl/fl DF vs. NF: Interaction F_(17,252) = 0.2886, p = 0.9978, time F_(17,252) = 2.514, p = 0.0011, group F_(1,252) = 24.82, p < 0.0001. Fxr^ΔHep DF-LPS N = 7, Fxr^ΔHep NF-LPS N = 5, Fxr^fl/fl DF-LPS N = 8, Fxr^fl/fl NF-LPS N = 8. Data are presented as mean values ± SEM.