a Changes in CypD, phospho-AMPK (Thr172), total AMPK, phospho-Akt (Ser473), and total Akt protein expression in the skeletal muscle of rats in protocol 1. β-actin served as a loading control. b–d Quantification of CypD immunoblots, phospho/total AMPK, and phospho/total Akt. The data are shown as the mean ± SD. #P < 0.05 and ##P < 0.01 vs. saline group, *P < 0.05 vs. intralipid group, $$P < 0.01 vs. BBR group; n = 3.