Table 1.

Top ten gene sets enriched to a greater extent in Chronic Adolescent Stress (CAS) rats compared to Non-Stressed (NS) rats following an acute immune challenge with LPS in adulthood.

Gene set	Female CAS		Female NS		ΔNES
	NES	FDR	NES	FDR	(CAS - NS)
ADIPOGENESISa	1.52	0.021	0.83	1	0.69
APICAL_JUNCTIONa	1.55	0.018	0.93	0.672	0.62
ESTROGEN_RESPONSE_EARLY	1.34	0.1	0.87	0.758	0.47
TGF_BETA_SIGNALING	1.43	0.049	0.98	0.744	0.45
MYOGENESIS*	1.35	0.098	0.95	0.671	0.4
PID_REG_GR_PATHWAY	1.88	0.001	1.57	0.015	0.31
UV_RESPONSE_UP	1.65	0.009	1.37	0.079	0.28
TNFA_SIGNALING_VIA_NFKBa	2.71	0	2.48	0	0.23
MYC_TARGETS_V2a	1.51	0.024	1.29	0.142	0.22
P53_PATHWAY	1.86	0.001	1.72	0.003	0.14

Gene set	Male CAS		Male NS		ΔNES
	NES	FDR	NES	FDR	(CAS - NS)
ADIPOGENESISa	1.62	0.009	0.59	1	1.03
MYOGENESISa	1.83	0.001	0.95	0.709	0.88
MYC_TARGETS_V2a	1.58	0.013	0.93	0.727	0.65
INTERFERON_GAMMA_RESPONSE	3.6	0	3.12	0	0.48
TNFA_SIGNALING_VIA_NFKBa	2.87	0	2.45	0	0.42
KRAS_SIGNALING_UP	1.94	0.001	1.52	0.027	0.42
GLYCOLYSIS	1.45	0.035	1.07	0.505	0.38
XENOBIOTIC_METABOLISM	1.84	0.001	1.49	0.035	0.35
APICAL_JUNCTIONa	1.66	0.007	1.31	0.114	0.35
INTERFERON_ALPHA_RESPONSE	3.27	0	2.95	0	0.32

Using the Molecular Signatures Database’s Hallmark (H) gene set in Gene Set Enrichment Analysis (GSEA), differential enrichment of 50 “hallmark” pathways representing well-defined biological processes as well as a gene set for glucocorticoid receptor regulatory network from Pathway Interaction Database (NCI, NIH and Nature Publishing Group) was assessed between LPS-challenged and saline-treated rats of Female CAS, Female NS, Male CAS, and Male NS backgrounds. Of the significantly enriched gene sets, Top 10 pathways with the largest difference in NES (ΔNES) between CAS and NS groups are shown for each sex.

CAS chronic adolescent stress, NS non-stressed, NES normalized enrichment score, FDR false discovery rate.

^aPathways whose enrichment is exaggerated by CAS in both males and females.