Fig. 3. Inhibition of miR-99b-3p suppresses Ang II-induced fibrotic responses in CFs.
Cultured CFs were treated with miR-99b-3p inhibitor or its negative control (NC inhibitor). Cells were further stimulated with or without 100 nM Ang II for 48 h. a–d Western blotting was conducted to detect the protein expression of fibronectin, collagen I, vimentin, and α-SMA. e The proliferation of CFs was measured by EdU incorporation assay. f A scratch-wound assay was used to test the cell migration ability. All data are presented as the mean ± SEM. *P < 0.05 vs. the NC group, #P < 0.05 vs. the NC plus Ang II treatment group, n = 3.