Fig. 1. Characterization of a novel PGK1 small molecule inhibitor.

a Flow chart for the high-throughput screening of PGK1 kinase inhibitors. b Chemical structure of NG52. c IC₅₀ determination of NG52 with purified PGK1 using the ADP-Glo assay, n = 3. d The effect of NG52 on purified PGK1 phosphatase activity. The NG52 dosage is indicated, n = 3. e The mode of inhibition of NG52 with PGK1 was examined using the ADP-Glo assay with the indicated concentration of ATP, n = 3. f Molecular modeling of PGK1 and NG52 binding. The results are the mean ± SD of three independent experiments; ns not statistically significant; ^***P < 0.001.