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. 2020 Jul 23;42(4):624–632. doi: 10.1038/s41401-020-0445-z

Fig. 2. The transcription factor EB promotes the tolerance of cells after drug treatment.

Fig. 2

a Immunofluorescence staining of TFEB in the LV-TFEB-MCF-7 cells. b Western blot detection of TFEB. c Western blot detection of cathepsin L in the LV-TFEB-MCF-7 cells. d Western blot detection of cathepsin D in the LV-TFEB-MCF-7 cells. e CCK-8 assay results showing the proliferation of LV-TFEB-MCF-7 cells treated with doxorubicin for 72 h. f Western blot detection of TFEB in the Si-TFEB-MCF-7 cells. g Western blot detection of the cathepsin L level in the Si-TFEB-MCF-7 cells. Actin was used as the loading control. h CCK-8 results showing the proliferation of the TFEB-knockdown cells treated with doxorubicin for 72 h. Quantitative analysis was performed with ImageJ software. Values are presented as the means ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ns P > 0.05 versus the control group.