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. 2020 Jul 23;42(4):624–632. doi: 10.1038/s41401-020-0445-z

Fig. 3. Artesunate inhibits the activity of cathepsins in MCF-7 cells.

Fig. 3

a Western blot detection of cathepsin L in the MCF-7 cells after treatment with artesunate for 24 h. b Western blot detection of cathepsin D in the MCF-7 cells after treatment with artesunate for 24 h. c LysoTracker detection in the MCF-7 cells after treatment with artesunate for 24 h. d Electron microscopy results showing autophagy in the MCF-7 cells treated with artesunate for 24 h. The black arrow indicates autophagosomes, and the red arrow indicates autolysosomes. e Western blot detection of P62 and LC3 in the MCF-7 cells after treatment with artesunate for 24 h in the presence or absence of bafilomycin A1. Actin was used as the loading control. Quantitative analysis was performed with ImageJ software. Values are presented as the means ± SD from three independent experiments. *P < 0.05, ***P < 0.001, and ns P > 0.05 versus the control group.