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. 2021 May 12;12(5):474. doi: 10.1038/s41419-021-03749-x

Fig. 8. NF-κBp65/PUMA-regulated hepatocytes apoptosis-linked inflammatory response to promote HSCs activation and liver fibrosis.

Fig. 8

a The area of the indicated inflammatory cytokines from immunofluorescence staining of Fig. 7c was analyzed. *P < 0.05 versus p65f/f mice, #P < 0.05 versus p65Δhepa mice with vehicle administration. b The administration of TGF-β1 inhibitor pirfenidone, TNF-α inhibitor pentoxifylline or monocytes inactivator clodronate-loaded liposomes, attenuated HSCs activation (α-SMA staining of primary HSCs, red) and liver fibrosis (α-SMA staining, red) in p65Δhepa/PUMA-WT mice. Nuclei (blue) were counterstained with DAPI, n = 6 per group. IF, immunofluorescence staining. c, d Western blotting presented that pirfenidone, pentoxifylline, or clodronate-loaded liposomes attenuated liver fibrosis (liver section) and the activation of HSCs (primary HSCs section) in CCl4-induced p65Δhepa/PUMA-WT mouse model, respectively. n = 6 per group.