Self-regeneration and time-dependent cell viability of EC-SLM. (A) Optical images of first (Gen I), second (Gen II) and third (Gen III) generations of EC-SLM. A small fragment (dotted rectangle) of Gen I was cultured, pelletized and air-dried to produce the Gen II, which in turn resulted in Gen III. Scale bar 0.5 cm. (B) CFU count of Gen II and Gen III of EC-SLM. n=6. The graph shows mean values, and the error bars are standard deviation. (C) Young’s modulus and (D) Hardness of Gen II and Gen III EC-SLMs measured by nanoindentation (n≥125). The graphs show median and the range. (E) Time dependent CFU analysis of EC-SLM. n=6. The graph shows mean values, and the error bars are standard deviation.