Fig. 5. SARS-CoV-2 infection influences m⁶A methylome of Huh7 cell transcripts.

a Distribution of the enriched m⁶A peaks in uninfected (blue line) and SARS-CoV-2-infected Huh7 cells (red line) analyzed along the RNA segments. Each segment was normalized according to its average length in Refseq annotation. Barplot shows the percentage of m⁶A peaks in the indicated regions. b Charts showing the distribution of gained (top) and lost m⁶A peaks (bottom) in the 5′ UTR (orange), CDS (blue), 3′ UTR (red) and ncRNA (green) of host cell RNA transcripts. Huh7 cells were uninfected or SARS-CoV-2 infected, and m⁶A peaks in total cellular RNA were analyzed at 120 h after infection. Representative of n = 2 determinations. c MA plot showing changes in m⁶A peak intensity of interferon-stimulated genes before and after SARS-CoV-2 infection. Upon SARS-CoV-2 infection, interferon-stimulated genes with no obvious changes in m⁶A peak intensity, higher m⁶A peak intensity and lower m⁶A peak intensity are represented by gray dots, red dots and blue dots, respectively. d GO enrichment analyses of genes with downregulated m⁶A peaks (left) and upregulated m⁶A peaks (right). e Motif analysis to identify consensus sequences for lost m⁶A peaks (left) and gained m⁶A peaks (right). The top four motifs for each are shown.