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. 2020 Jul 21;42(4):560–572. doi: 10.1038/s41401-020-0458-7

Fig. 3. A decrease in [Cl−]i attenuates VEGF-induced cytoskeletal reorganization in HUVECs.

Fig. 3

The cells were treated with normal Cl− or low Cl− medium containing VEGF (50 ng/mL) for 48 h. F-actin was visualized by rhodamine-phalloidin staining. Focal adhesions in HUVECs were observed by immunofluorescence staining using paxillin and vinculin antibodies, respectively. Representative images from five independent experiments are shown. White arrows indicate stress fibers and focal adhesions. Scale bars = 50 µm, n = 6.