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. 2021 May 12;11:9749. doi: 10.1038/s41598-021-88870-9

Figure 3.

Figure 3

Effect of the c-9, t-11-CLA diet on the number of microglia and astrocytes in the brain of AD model mice. (A) Immunostaining of the brain sections of c-9, t-11-CLA diet-fed and control diet-fed AD model mice with anti-GFAP (astrocyte marker) and anti-IBA-1 antibodies (microglia marker). The number of IBA-1 (green)-positive signals was significantly increased in the cortex and hippocampus of c-9, t-11-CLA diet-fed mice compared with the control (right panels), whereas the number of GFAP (red)-positive signals was not changed (left panels). (B) The histograms show the numbers of GFAP-positive and IBA-1-positive signals (n = 6 mice for each group; five different fields per mouse were used to count the number). GFAP: glial fibrillary acidic protein; IBA-1: ionized calcium binding adaptor molecule 1. Bar, 20 µm. **P < 0.01 (cortex, P = 7.3 × 10−8; hippocampus, P = 3.8 × 10−5).