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. 2021 May 12;11:9749. doi: 10.1038/s41598-021-88870-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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LC–MS/MS analysis of synthetic sn-1- and sn-2-CLA-LPC. Synthetic sn-1-CLA-LPC (top panel), synthetic sn-2-CLA-LPC (second panel from the top), the LPC peak area of the cortex lysate from control diet-fed mice (third panel from the top), and the LPC peak area of the cerebellum lysate from CLA diet-fed mice (bottom panel) were detected with LC–MS/MS analysis. Synthetic sn-1-CLA-LPC corresponded to the extra peak with a delayed retention time following the two peaks of LA-LPC (sn-1-LA and sn-2-LA. See²⁷) observed in the cortex, whereas the peak of sn-2-CLA-LPC corresponded to that of sn-1-LA-LPC.