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. 2021 May 12;12:2742. doi: 10.1038/s41467-021-22953-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Mean and individual b melanoma spheroid invasion (Kruskal–Wallis with Dunn’s multiple comparison tests *p = 0.0317, ****p < 0.0001, n = 8 replicate spheroids for three cell lines across two-independent experiments), and c single cell invasion (Skmel28) (Kruskal–Wallis with Dunn’s multiple comparison tests ****p < 0.0001, n = 9 independent measurements over two experiments) in different collagen concentrations. d Representative images of spheroid and melanoma single cell invasion, top: Hoechst (inverted), bottom: invading cells (blue). e A375 melanoma invasion H&E (scale bar: 10 µm) and f single cell invasion in organotypic dermal collagen HFF and UV-HFF constructs (two-sided Mann–Whitney U *p = 0.0485, ns not significant, data represents 12 fields of view across two-independent experiments). g Mean and individual h melanoma invasion (Kruskal–Wallis with Dunn’s multiple comparison tests ***p < 0.001, ****p < 0.0001, n = 8 replicate spheroids for three cell lines across two-independent experiments), and i melanoma single cell invasion (Kruskal–Wallis with Dunn’s multiple comparison tests *p = 0.0426, n = 7 independent measurements over two experiments) by collagenase I concentration. j Representative images of spheroid and single cell invasion, top: Hoechst (inverted), bottom: invading cells (blue). k Melanoma spheroid invasion by adult patient fibroblast secretome, MMP1 levels (two-sided Pearson correlation, red: Sk-mel-28 R = −0.39 p = 0.1, blue: A375 R = −0.21 p = 0.4, data represent eight independent cell lines measured in duplicate) and l by COL1A1 relative expression (RE) in fibroblasts (two-sided Pearson correlation, red: Sk-mel-28 R = 0.57 p = 0.02, blue: A375 R = 0.32 p = 0.2, data represent eight independent cell lines measured in duplicate). Error bars: standard error of the mean (bar).