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. 2021 May 12;4:568. doi: 10.1038/s42003-021-02083-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Shown are ATAC-seq and ChIP-seq data indicating that NKX2-1 binds to far distal (#1), distal (#2) and proximal (#3) upstream regions and the third (#4), and sixth (#5) intronic regions of the human MYBPH locus. Significant peaks are described as in Fig. 2a. b Shown is the gene expression of NKX2-1 downstream target genes affected by CRISPRi as described in Fig. 2b. The sgRNA targeting NKX2-1-binding sites located at three upstream (#1, #2, and #3) and one intronic (#4) regions significantly suppressed the expression of MYBPH. The sgRNA targeting NKX2-1-binding sites located at the sixth intronic (#5) region also significantly suppressed the expression of SFTPA1 induced by NKX2-1 in A549 cells. Results are expressed as the mean ± SD of the triplicates for each group. Only P < 0.05 and more than twofold suppression are considered significant CRISPRi-mediated suppression (highlighted in red). Unt. untransfected, Cont. non-targeted control sgRNA.