Fig. 6. NKX2-1-binding sites located at three upstream and one intronic region are commonly required for the expression of MYBPH.
a Shown are ATAC-seq and ChIP-seq data indicating that NKX2-1 binds to far distal (#1), distal (#2) and proximal (#3) upstream regions and the third (#4), and sixth (#5) intronic regions of the human MYBPH locus. Significant peaks are described as in Fig. 2a. b Shown is the gene expression of NKX2-1 downstream target genes affected by CRISPRi as described in Fig. 2b. The sgRNA targeting NKX2-1-binding sites located at three upstream (#1, #2, and #3) and one intronic (#4) regions significantly suppressed the expression of MYBPH. The sgRNA targeting NKX2-1-binding sites located at the sixth intronic (#5) region also significantly suppressed the expression of SFTPA1 induced by NKX2-1 in A549 cells. Results are expressed as the mean ± SD of the triplicates for each group. Only P < 0.05 and more than twofold suppression are considered significant CRISPRi-mediated suppression (highlighted in red). Unt. untransfected, Cont. non-targeted control sgRNA.