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. 2020 Nov 23;46(6):1183–1193. doi: 10.1038/s41386-020-00912-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative prelimbic layer II/III spontaneous excitatory postsynaptic current (sEPSC) traces recorded following behavioral testing for mice tested alone or with a stranger and either treated with saline (black) or metyrapone (red). A representative trace for the cagemate condition is also shown. b Representative prelimbic layer II/III spontaneous inhibitory postsynaptic current (sEPSC) traces recorded following behavioral testing for mice tested alone or with a stranger and either treated with saline (black) or metyrapone (red). A representative trace for the cagemate condition is also shown. c sEPSC frequency (Hz) is enhanced in stranger dyads compared with mice tested alone and cagemate dyads (one-way ANOVA, F_2,30 = 6.42, ^†p < 0.05, ^††p < 0.01 compared with stranger dyads, all vehicle-treated conditions). sEPSC frequency (Hz) is normalized by metyrapone pre-treatment in stranger dyads (two-way ANOVA, main effect of drug: F_1,36 = 3.43, p = 0.07; main effect of social context: F_1,36 = 4.25, p < 0.05; social context × drug interaction: F_1,36 = 5.16, p < 0.05). d sEPSC amplitude (pA) is not significantly different between vehicle-treated mice tested alone or within a stranger or cagemate dyad (one-way ANOVA, F_2,30 = 1.90, p > 0.05). Metyrapone pre-treatment did not alter sEPSC amplitude (pA) in mice tested alone or within a stranger dyad (two-way ANOVA, main effect of drug: F_1,36 = 1.14, p > 0.05; main effect of social context: F_1,36 = 0.65, p > 0.05; social context x drug interaction: F_1,36 = 1.90, p > 0.05). e sIPSC frequency (Hz) is not significantly different between vehicle-treated mice tested alone or within a stranger or cagemate dyad (one-way ANOVA, F_2,30 = 0.05, p > 0.05). Metyrapone pre-treatment did not alter responses in mice tested alone or within a stranger dyad (two-way ANOVA, main effect of drug: F_1,36 = 0.40, p > 0.05; main effect of social context: F_1,36 = 2.12, p > 0.05; social context × drug interaction: F_1,36 = 1.36, p > 0.05). f sIPSC amplitude (pA) is not significantly different between vehicle-treated mice tested alone or within a stranger or cagemate dyad (one-way ANOVA, F_2,30 = 1.39, p > 0.05). Metyrapone pre-treatment enhanced sIPSC amplitude (pA) compared with mice tested alone and vehicle-treated stranger dyads (two-way ANOVA, main effect of drug: F_1,36 = 1.83, p > 0.05; main effect of social context: F_1,36 = 10.57, p < 0.01; social context × drug interaction: F_1,36 = 9.44, p < 0.01). g Synaptic drive is increased in stranger dyads compared with mice tested alone or within a cagemate dyad (one-way ANOVA, F_2,30 = 8.80, ^†††p < 0.01 compared with stranger dyads, all vehicle-treated conditions). Synaptic drive is normalized by metyrapone pre-treatment in stranger dyads (two-way ANOVA, main effect of drug: F_1,36 = 17.15, p < 0.001; main effect of social context: F_1,36 = 6.97, p = 0.012; social context × drug interaction: F_1,36 = 6.01, p = 0.019). Similar to Fig. 2, a metyrapone-treated cagemate condition was not included based on previous work [11]. n = 9–11 cells/group prepared from 4 to 5 mice. *p < 0.05, **p < 0.01, ***p < 0.001.