Skip to main content
. 2020 Aug 4;42(5):744–754. doi: 10.1038/s41401-020-0477-4

Fig. 7. DC-STAMP-siRNA abolished the inhibitory effects of ORI on osteoclast formation and NFATc1 expression.

Fig. 7

We synthesized four target siRNA sequences and verified their knockdown efficiency by a, b qPCR and c, d WB; the siRNA with the highest knockout efficiency was used for the follow-up experiments. e RAW264.7 cells were seeded and transfected with siRNA-DC-STAMP (40 nM) using Lipofectamine RNAIMAX. After ~24 h of siRNA interference, the cells were stimulated by RANKL with or without ORI (1.56 μM). The media were changed every 2 days. After 4 days, the cells were stained with TRAP. f TRAP+ multinucleated cells (>3 nuclei) were counted as mature OCs. g RAW264.7 cells stably expressing the NFAT-luc luciferase reporter construct were transfected with siRNA-DC-STAMP for 24 h and then treated with ORI (1.56–3.125 μM) and RANKL (100 ng/mL) for 24 h. The luciferase activity was measured to indicate NFATc1 transcription. a–f *P < 0.05, ***P < 0.001 vs the si-NC group. g ###P < 0.001 vs the si-NC group; *P < 0.05, ***P < 0.001 vs the RANKL-treated group.