Skip to main content
. 2021 May 12;12:2763. doi: 10.1038/s41467-021-23035-w

Fig. 3. Rif2 BAT blocks 5′ resection and MRX complex presence at broken ends.

Fig. 3

A Rif2 N-terminal region stabilises broken ends. Left panel: the stability of I-SceI-induced broken ends with 5 Gal4 sites determined by Southern blot in G1 and G2/M arrested cells. Right panel: quantification of the uncut and cut signals normalised to the control ADE1 signal. Means from independent samples. Experiment reproduced three times. B Mre11 and Xrs2 presence at I-SceI-induced broken ends with 5 Gal4 sites determined by ChIP in G1 arrested cells. Quantification of immunoprecipitated DNA (IP) relative to the input DNA (IN). Means from independent samples. Quantification of I-SceI cleavage efficiency in Supplementary Fig. 4D.