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. 2021 May 12;12:2763. doi: 10.1038/s41467-021-23035-w

Fig. 6. Identification of Rad50 residues essential for BAT function and interaction.

Fig. 6

A Position of the eight residues (red) selected with CABSdock as potentially interacting with Rif2 BAT. B 2-Hybrids interactions between mutant full-length Rad50 and Rif2 N-terminal region or full-length Mre11. C GST pull-down interaction between Rif2 N-terminal region and Rad50 ATPase Head. Experiment reproduced three times. D NHEJ inhibition by Rif2 BAT motif at I-SceI-induced broken ends in rad50 mutants (single I-SceI site assay (Supplementary Fig. 2B)). Means from independent cell cultures. E Impact on telomere length of mutants rad50-K6A and rad50-K81E in WT cells and in cells lacking Rif1 or Rif2 (Southern blot, Y′ probe, XhoI digest). Experiment reproduced three times.