Table 1.
Targeted quantitative analyses of selected proteins using isotopically-labeled (15N) peptide standards.
| Target proteina | 15N-Peptide sequence | Peptide concentration (copies cell −1) | |
|---|---|---|---|
| atm. O2 | low O2 | ||
| Nitrite oxidoreductase, alpha subunit 1 | LVVITPEYNPTAYR | 845 (52) | 1924 (275) |
| DYAFPDFANSYSGK | 241 (40) | 451 (91) | |
| HPFWEETNESKPQWTR | 673 (39) | 1571 (388) | |
| Nitrite oxidoreductase, alpha subunit 2 | VVVITPEYNPTAQR | 1,154 (27) | 851 (187) |
| Nitrite oxidoreductase, alpha subunit 3 | DYQFPDFTSTYSGK | 1,761 (159) | 1,261 (242) |
| SGIDPALTGTHR | 4,519 (516) | 3,400 (526) | |
| IAVITPEYNPTAYR | 4,860 (496) | 3,741 (710) | |
| Nitrite oxidoreductase, alpha subunit (all) | GWKPSDPYYK | 11,467 (1,957) | 9,564 (1,083) |
| AIALDTGYQSNFR | 13,996 (962) | 13,116 (2,934) | |
| Pyruvate:ferredoxin oxidoreductase, | TPSFFTGSEVIK | 2,026 (192) | 1,550 (248) |
| alpha subunit | EAIAILEEEGIR | 1,362 (80) | 1,054 (170) |
| EVSATVPNNER | 2,453 (169) | 1,736 (215) | |
| Ribonucleotide reductase | TGESPYQTIPFSHR | 300 (46) | 198 (30) |
| EAAVPEPYIHR | 705 (132) | 472 (77) | |
| IINQSLPPALR | 750 (88) | 546 (68) | |
Nitrite oxidoreductase peptide targets were designed to match a specific NxrA copy (1, 2, or 3), or to match all three as indicated in the target protein column. Values represent the mean of triplicate measurements and standard deviations are shown in brackets.
aA complete list of quantified proteins can be found in Data Set 2.