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. 2021 May 12;4:563. doi: 10.1038/s42003-021-02079-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative conventional flow cytometry plots from an adult tonsil showing the hierarchical phenotype gating strategy from singlet lymphocytes to the ILC1 (green), ILC2 (pink), and ILC3 (blue) and a unique ILC subset (ILC_FR) (red) populations indicated by arrows and color-coded gates. (Lin = CD4, CD11b, CD11c, CD14, CD16). b Graph shows frequency of ILC1, ILC2, ILC3, and ILC_FR among CD45⁺ human tonsil mononuclear cells (n = 7 or 8 biologically independent tonsils per group, 3 independent experiments) (Ordinary one-way ANOVA with multiple comparisons; F = 5.2; **p = 0.0058; Mean ± SD). c Analysis of surface and transcription markers on ILC_FR by flow cytometry. Gray histograms depict fluorescence minus one (FMO) control of each antibody. Red lines indicate ILC_FR. Blue lines denote ILC1 (for CD56, Tbet), ILC2 (KLRG1, GATA3), ILC3 (NKp44, ID3, CD127, ILR12RB1, CD7, CCR6, IL-2Rγ), and T cells (CD4) (n = 10 biologically independent tonsils per group, 3 independent experiments).