Fig. 2. The effects of C24 on lipogenesis in vitro and in vivo.

a The effect of ¹⁴C-labeled de novo lipogenesis in HepG2 cells after different doses of C24 treatment. b, c Western blotting analysis (b) and relative integrated density (c) of the indicated proteins in livers of C57BL/6J mice after 2 h of treatment with or without C24 (100 mg · kg⁻¹) (n = 6). d The effect of ¹⁴C-labeled de novo lipogenesis in liver of fasted-refed mice after 2 h of treatment (n = 4). e, f After tyloxapol (600 mg · kg⁻¹) tail vein injection, 0–4 h of the plasma TG concentration (e) and the area under curve (AUC) (f) were measured in high-carbohydrate diet-fed C57BL/6J mice after 3 d of treatment (n = 9/10). Data are presented as the means ± S.D. Multiple groups were analyzed using one-way ANOVA. Two groups were analyzed using two-tailed unpaired Student’s t test or two-way ANOVA, *P < 0.05 vs. control; **P < 0.01 vs. control; ***P < 0.001 vs. control.