Fig. 3. C24 inhibits lipogenesis via AMPK pathway.

a, b Western blotting analysis of the indicated proteins (a) and ¹⁴C-labeled de novo lipogenesis (b) in HepG2 cells after compound C and C24 treatment. CC represents compound C, ns means no significant difference. c, d Western blotting analysis of the efficiency of AMPKα knockout and effects of C24 on AMPK activation in hepatocytes isolated from AMPK FLOX and AMPK LKO mice. e, f Effects of C24 on lipogenesis in hepatocytes isolated from AMPK FLOX (e) and AMPK LKO (f) mice. Data are presented as the means ± S.D. Multiple groups were analyzed using one-way ANOVA, two groups were analyzed using two-tailed unpaired Student’s t test, *P < 0.05 vs. control; **P < 0.01 vs. control.