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. 2020 Dec 4;7(1):mgen000491. doi: 10.1099/mgen.0.000491

Table 2.

Adjusted thresholds for optimised clustering concordance between cgMLST methods and between cgMLST and SNP methods. Clustering by cgMLST methods at published thresholds [12, 13] (in bold type on the left) was set as reference for the adjustment of clustering thresholds for other methods. The columns show the different comparison methods and the threshold values (alleles or SNPs) at which the greatest possible agreement among the clustering with the respective reference method was achieved based on adjusted Wallace coefficients presented in Fig. 5. As cluster comparison is direction-dependent, the table must be read from the left to the right

cgMLST

SNP

General reference

MLST CC-specific reference

Allele threshold

Ridom_Ruppitsch

chewBBACA_Ruppitsch

BioNumerics_Moura

Snippy_EGDe

BioNumerics_EGDe

Snippy_closed

Snippy_draft

BioNumerics_closed

BioNumerics_draft

cgMLST

Ridom_

Ruppitsch

7

6

6

9–10

4

11

12

12

11–12

chewBBACA_

Ruppitsch

7

7

9–10

4

11

12

12

11–12

BioNumerics_

Moura

8

7

13

4

12

12

11

11–12

Ridom_

Ruppitsch

10

10

10

15

5

19

19

18

18

chewBBACA_

Ruppitsch

10

10

15

5

20

18–19

18

18

BioNumerics_

Moura

11

11

18

6

20

22

19

20