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. 2021 Apr 28;12:641210. doi: 10.3389/fphar.2021.641210

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Copyright © 2021 Cocetta, Governa, Borgonetti, Tinazzi, Peron, Catanzaro, Berretta, Biagi, Manetti, Dall’Acqua and Montopoli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of CBD and THC extracts (0.1 and 1 μg/mL) on occludin and ZO-1 expression in Caco-2 cells. (A) Representative images of the effect of CBD-THC treatment on tight junction proteins in Caco-2 cell monolayers. (B) Representative images of the effect of CBD and THC on tight junction proteins in inflammatory conditions (INFγ+TNFα stimulation). Images were collected by confocal laser-scanning microscope LSM800 and software ZEN 2.1, magnification 60X and are representative of three experiments.