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. 2020 Nov 18;3(1):acmi000179. doi: 10.1099/acmi.0.000179

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© 2021 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 3. — The distributions of pEK204-like regions in ST131 Clade C genome assemblies with >1 Kb (left, n=3234) and >40 Kb (right, n=193) of matching segments. The regions of similarity were examined in (left) Subclades B0 (n=14 in black), C0 (n=51 in red), C1 (n=1119 in green) and C2 (n=2051 in blue), and (right) C0 (n=17 in red), C1 (n=82 in green) and C2 (n=94 in blue). The 8289_1#27 (c2) is highlighted in yellow. Similarity across pEK204 (93 732 bp) was based on blast alignment. The bla _CTX-M gene was at 8 Kb, bla _TEM was at 13 Kb, followed by a mix of conjugation and segregation genes at 36–70 Kb. This suggested independent integrations and rearrangements of pEK204-like plasmids across the subclades. The annotation was modified from [59].