Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 12;12(5):e00356. doi: 10.14309/ctg.0000000000000356

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Study design overview. (a) Selection of candidate miRNAs using the organoid culture. Organoids from the normal colon crypts and CRA are established in pairs from endoscopically resected specimens. Then, the exosomes are isolated from conditional medium collected from each organoid using ultracentrifugation. Then, microarray analysis is conducted for each of the exosomal miRNAs isolated from the normal colon crypt-derived organoids and CRA-derived organoids. CRA-specific miRNA candidates are extracted. (b) Flow diagram showing the strategy for qRT-PCR analysis of serum miRNAs before and after endoscopic resection. Pretreatment sera are obtained from patients with CRA. Then, CRA is resected using ESD within 1 month. Posttreatment sera are obtained 6 months after resection. At that time, total colonoscopy has been performed to confirm healing of the mucosa and lack of recurrence or residual tumor in the treated area. The expression changes between pretreatment sera and posttreatment sera of candidate miRNAs are validated using RT-PCR. CRA, colorectal adenoma; miRNAs, microRNAs; ESD, endoscopic submucosal dissection; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.