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. 2021 May 12;12(5):e00356. doi: 10.14309/ctg.0000000000000356

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© 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Patient-derived organoids and isolation of exosomes. (a) Top panels show the images of normal colon crypts. Optical microscope image of organoids (left), H&E staining of organoids (center), and H&E staining of endoscopically resected specimens (right). Bottom panels show the images of the CRA. Optical microscope image of organoids (left), H&E staining of organoids (center), and H&E staining of endoscopically resected specimens (right). Each scale bar is 100 μm. (b) Electron microscope imaging of the isolated exosomes. Images of exosomes from normal colon crypt-derived organoids (left) and of exosomes from CRA-derived organoids (right). Original magnification: ×15,000. Scale bar shows 100 nm. (c) Size distribution of isolated exosomes analyzed using a Nanosight NS300. Size distribution of normal colon crypt-derived organoids (left). Size distribution of exosome from CRA-derived organoids (right). (d) Exosomal miRNAs are prepared from normal crypt-derived and CRA-derived organoids. Their integrity and quality are examined using a bioanalyzer system. Exosomal miRNA did not include ribosomal RNAs. Image of exosomal miRNAs from normal colon crypt-derived organoids (left), image of exosomal miRNAs from CRA-derived organoids (right). CRA, colorectal adenoma; H&E, hematoxylin–eosin; miRNA, microRNA.