| Gene (Homo sapiens) |
DCC |
NCBI |
1630 |
|
| Gene (Mus musculus) |
Dcc |
NCBI |
13176
|
|
| Gene (Mus musculus) |
Ntn1 |
NCBI |
18208 |
|
| Strain, strain background (Mus musculus) |
Dccflox/flox, C57BL/6J |
Krimpenfort et al., 2012 |
N/A |
|
| Strain, strain background (Mus musculus) |
Dcckanga, C57BL/6J |
Finger et al., 2002 |
N/A |
|
| Strain, strain background (Mus musculus) |
Dcc-/-, C57BL/6J |
Fazeli et al., 1997 |
N/A |
|
| Strain, strain background (Mus musculus) |
Emx1iCre, C57BL/6J |
Kessaris et al., 2006 |
N/A |
|
| Strain, strain background (Mus musculus) |
Ntn1-lacZ, C57BL/6J |
Serafini et al., 1996 |
N/A |
|
| Strain, strain background (Mus musculus) |
tdTomatoflox_stop, C57BL/6J |
Madisen et al., 2010 |
N/A |
|
| Cell line (Homo sapiens) |
HEK293T |
ATCC |
RRID:CVCL_0045
|
ATCC Cat# CRL-1573 |
| Cell line (Homo sapiens) |
U251MG |
ATCC |
RRID:CVCL_0021
|
Obtained as U-373MG (RRID:CVCL_2219) but subsequently identified as U-251 via PCR-based short tandem repeat profiling |
| Cell line (Mus musculus) |
Neuro-2A (N2A) |
ATCC |
RRID:CVCL_0470
|
Obtained via the University of Queensland |
| Cell line (Chlorocebus aethiops) |
COS-7 |
ATCC |
RRID:CVCL_0224
|
ATCC Cat# CRL-1651 |
| Antibody |
Goat polyclonal anti-DCC |
Santa Cruz Biotechnology |
sc-6535, RRID:AB_2245770
|
(1:200) western blot; (1:500)immunofluorescence |
| Antibody |
Goat polyclonal anti-NTN1 |
R&D Systems |
AF1109, RRID:AB_2298775
|
(1:500) western blot; (1:500) immunofluorescence |
| Antibody |
Rabbit monoclonal anti-GADPH |
Cell Signaling Technology |
2118, RRID:AB_561053
|
(1:2000) western blot |
| Antibody |
Rabbit polyclonal anti-GADPH |
IMGENEX |
IMG-5143A, RRID:AB_613387
|
(1:1000) western blot |
| Antibody |
Rabbit polyclonal anti-APC |
Abcam |
ab15270, RRID:AB_301806
|
(1:250) |
| Antibody |
Mouse monoclonal anti-α-DAG1 |
Merck |
05-593, RRID:AB_309828
|
(1:250) |
| Antibody |
Rabbit polyclonal anti-β-catenin |
Cell Signaling Technology |
9562, RRID:AB_331149
|
(1:500) |
| Antibody |
Mouse monoclonal anti-β-dystroglycan (MANDAG2) |
Developmental Studies Hybridoma Bank |
7D11, RRID:AB_2211772
|
(1:50) |
| Antibody |
Chicken polyclonal anti-β-galactosidase |
Abcam |
ab9361, RRID:AB_307210
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-cleaved-caspase 3 |
Cell Signaling Technology |
9661, RRID:AB_2341188
|
(1:500) |
| Antibody |
Goat polyclonal anti-DCC |
Santa Cruz Biotechnology |
sc-6535, RRID:AB_2245770
|
(1:500) |
| Antibody |
Mouse monoclonal anti-GAP43 |
Millipore |
MAB347, RRID:AB_94881
|
(1:500) |
| Antibody |
Mouse monoclonal anti-GFAP |
Millipore |
MAB3402, RRID:AB_94844
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-GFAP |
Dako |
Z0334, RRID:AB_10013382
|
(1:500) |
| Antibody |
Mouse monoclonal anti-Glast (EAAT1) |
Abcam |
Ab49643, RRID:AB_869830
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-Glast (EAAT1) |
Abcam |
Ab416, RRID:AB_304334
|
(1:250) |
| Antibody |
Mouse monoclonal anti-KI67 |
BD Pharmingen |
550609, RRID:AB_393778
|
(1:500) |
| Antibody |
Chicken polyclonal anti-Laminin |
LS-Bio |
C96142, RRID:AB_2033342
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-Laminin (pan-Laminin) |
Sigma |
L9393, RRID:AB_477163
|
(1:500) |
| Antibody |
Mouse monoclonal anti-N-cadherin (CDH2) |
BD Biosciences |
610921, RRID:AB_398236
|
(1:250) |
| Antibody |
Rat monoclonal anti-Nestin (NES) |
Developmental Studies Hybridoma Bank |
AB 2235915, RRID:AB_2235915
|
(1:50) |
| Antibody |
Chicken polyclonal anti-Nestin (NES) |
Abcam |
Ab134017, RRID:AB_2753197
|
(1:1000) |
| Antibody |
Goat polyclonal anti-NTN1 |
R&D Systems |
AF1109, RRID:AB_2298775
|
(1:500) |
| Antibody |
Mouse monoclonal anti-neurofilament |
Millipore |
MAB1621, RRID:AB_94294
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-NFIA |
Aviva Systems Biology |
ARP32714, RRID:AB_576739
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-NFIB |
Sigma |
HPA003956, RRID:AB_1854424
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-neuronal-specific-ßIII-tubulin (TUBB3) |
Abcam |
Ab18207, RRID:AB_444319
|
(1:500) |
| Antibody |
Rabbit polyclonal anti-phospho p44/42 MAPK (ERK1/2) |
Cell Signaling Technology |
9101, RRID:AB_331646
|
(1:250) |
| Antibody |
Rabbit polyclonal anti-SOX9 |
Merck |
AB5535, RRID:AB_2239761
|
(1:500) |
| Antibody |
Goat polyclonal anti-TDTOMATO |
Sicgen |
Ab8181-200, RRID:AB_2722750
|
(1:500) |
| Recombinant DNA reagent |
pCAG-TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCC:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCKANGA:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCM743L:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCV754M:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCA893T:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCV793G:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCMG805E:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-DCCM1217V;A1250T:TDTOMATO |
This paper |
|
|
| Recombinant DNA reagent |
pCAG-H2B-GFP-2A-MyrTDTOMATO |
Arnold Kriegstein (UCSF) |
|
|
| Recombinant DNA reagent |
p-SUPER-Dcc-shRNA |
Xiong Zhiqi; Zhang et al., 2018
|
|
|
| Recombinant DNA reagent |
pCAG-Dcc-CRISPR 1 |
Atum; this paper |
|
Targeting chr18:71954969– 71955009 |
| Recombinant DNA reagent |
pCAG-Dcc-CRISPR 2 |
Atum; this paper |
|
Targeting chr18:71826146– 71826092 |
| Recombinant DNA reagent |
Fgf8 cDNA |
Gail Martin, UCSF |
|
In situ hybridization riboprobe |
| Recombinant DNA reagent |
Ntn1 cDNA |
Helen Cooper |
|
In situ hybridization riboprobe |
| Recombinant DNA reagent |
Mmp2 cDNA |
This paper |
|
In situ hybridization riboprobe |
| Sequence-based reagent |
Mmp-2 cDNA forward primer |
Allen Brain Atlas |
|
5’-ATGGTGACCAAGAACAGAAGGT |
| Sequence-based reagent |
Mmp-2 cDNA reverse primer |
Allen Brain Atlas |
|
5’-AATCACTGCTACAATCACCACG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Met743Leu forward primer |
This paper |
|
5’-GAGGAGGTGTCCAACTCAAGATGATACAGTTTGTCTG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Met743Leu reverse primer |
This paper |
|
5’-CAGACAAACTGTATCATCTTGAGTTGGACACCTCCTC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Val754Met forward primer |
This paper |
|
5’-TAATATAGCCTCTCACCATGATGTTTGGGTTGAGAGG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Val754Met reverse primer |
This paper |
|
5’-CCTCTCAACCCAAACATCATGGTGAGAGGCTATATTA |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Ala893Thr forward primer |
This paper |
|
5’- ACTTGTACTTGGTACTGGCAGAAAAGCTGGTCCT |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Ala893Thr reverse primer |
This paper |
|
5’-AGGACCAGCTTTTCTGCCAGTACCAAGTACAAGT |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Val793Gl forward primer |
This paper |
|
5’-ACTAGAGTCGAGTTCTCATTATGGAATCTCCTTAAAAGCTTTCAAC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Val793Gl reverse primer |
This paper |
|
5’-GTTGAAAGCTTTTAAGGAGATTCCATAATGAGAACTCGACTCTAGT |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Gly805Glu forward primer |
This paper |
|
5’-CACTTTCGTAGAGAGGGACCTCTTCTCCGGCATTGTTGAA |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Gly805Glu reverse primer |
This paper |
|
5’-TTCAACAATGCCGGAGAAGAGGTCCCTCTCTACGAAAGTG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Met1217Val;p.Ala1250Thr forward 1 primer |
This paper |
|
5’-GTTCCAAAGTGGACACGGAGCTGCCTGCGTC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Met1217Val;p.Ala1250Thr reverse 1 primer |
This paper |
|
5’-GACGCAGGCAGCTCCGTGTCCACTTTGGAAC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Met1217Val;p.Ala1250Thr forward 2 primer |
This paper |
|
5’-GTACAGGGATGGTACTCACAACAGCAGGATTACTGG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Met1217Val;p.Ala1250Thr reverse 2 primer |
This paper |
|
5’-CCAGTAATCCTGCTGTTGTGAGTACCATCCCTGTAC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Val848Arg forward primer |
This paper |
|
5’-CAGCCTGTACACCTCTTGGTGGGAGCATGGGGG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.Val848Arg reverse primer |
This paper |
|
5’-CCCCCATGCTCCCACCAAGAGGTGTACAGGCTG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.His857Ala forward primer |
This paper |
|
5’-ACCCTCACAGCCTCAGCGGTAAGAGCCACAGC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.His857Ala reverse primer |
This paper |
|
5’-GCTGTGGCTCTTACCGCTGAGGCTGTGAGGG |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.p.del-P3(Kanga) forward primer |
This paper |
|
5’-CCACAGAGGATCCAGCCAGTGGAGATCCACC |
| Sequence-based reagent |
Dcc site-directed mutagenesis p.p.del-P3(Kanga) reverse primer |
This paper |
|
5’-GGTGGATCTCCACTGGCTGGATCCTCTGTGG |
| Peptide, recombinant protein |
Ntn1 |
R&D Systems |
1109-N1 |
100 ng/mL |
| Peptide, recombinant protein |
NTN1-AP |
This paper |
|
Generated as supernatant from HEK293T as previously described in Zelina et al., 2014
|
| Commercial assay, kit |
Click-iT EdU Alexa Fluor 488 Imaging Kit |
Invitrogen |
C10337 |
|
| Commercial assay, kit |
QuickChange II Site-Directed Mutagenesis Kit |
Stratagene |
200524 |
|
| Software, algorithm |
Fiji |
Fiji |
RRID:SCR_002285
|
|
| Software, algorithm |
Prism |
GraphPad |
RRID:SCR_002798
|
|
| Software, algorithm |
Imaris |
Bitplane |
RRID:SCR_007370
|
|
